Rat MIP-1 alpha (Macrophage Inflammatory Protein 1 Alpha) CLIA Kit
The Rat MIP-1 alpha (Macrophage Inflammatory Protein 1 alpha) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of MIP-1 alpha levels in rat serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it suitable for a variety of research applications.MIP-1 alpha is a key chemokine involved in inflammation and immune response, playing a crucial role in recruiting and activating immune cells.
Dysregulation of MIP-1 alpha has been implicated in various inflammatory conditions, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.With its high sensitivity and specificity, the Rat MIP-1 alpha CLIA Kit provides researchers with a powerful tool for accurately quantifying MIP-1 alpha levels in rat samples, facilitating the study of immune response and inflammation in preclinical research settings.
Product Name:
Rat MIP-1 alpha (Macrophage Inflammatory Protein 1 Alpha) CLIA Kit
SKU:
RTES00369
Target:
Rat MIP-1 alpha (Macrophage Inflammatory Protein 1 Alpha)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat MIP-1α. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MIP-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MIP-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat MIP-1α. You can calculate the concentration of Rat MIP-1α in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-99
91-108
91-104
Average (%)
91
98
96
1:4
Range (%)
95-109
99-113
83-97
Average (%)
101
107
90
1:8
Range (%)
94-109
89-103
103-119
Average (%)
101
95
109
1:16
Range (%)
98-112
98-115
87-100
Average (%)
104
106
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-107
99
EDTA plasma (n=5)
88-101
95
Cell culture media (n=5)
93-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.68
69.55
169.72
27.72
63.98
167.98
Standard deviation
2.67
6.95
15.38
3.3
6.82
12.8
C V (%)
9.65
9.99
9.06
11.9
10.66
7.62
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat MIP-1α concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MIP-1α in samples. No significant cross-reactivity or interference between Rat MIP-1α and analogues was observed.
