Rat MHC1/AgB1/H-11 (Major Histocompatibility Complex1) CLIA Kit
The Rat MHC1 (AGB1-H-11) Major Histocompatibility Complex 1 CLIA Kit is specifically designed for the quantitative measurement of MHC1 levels in rat samples. This kit offers high sensitivity and specificity, allowing for accurate and reproducible results in serum, plasma, and cell culture supernatants.MHC1 plays a key role in the immune response by presenting antigens to T cells, making it a crucial component in understanding immune system function and response to disease.
By accurately measuring MHC1 levels, researchers can gain valuable insights into immune system dynamics, autoimmune diseases, and transplant rejection.With its reliable performance and wide range of applications, the Rat MHC1 CLIA Kit is a valuable tool for researchers studying immunology, transplantation, and autoimmune disorders. Get precise and reliable results with this innovative kit from Assay Genie.
Product Name:
Rat MHC1/AgB1/H-11 (Major Histocompatibility Complex1) CLIA Kit
SKU:
RTES00376
Target:
Rat MHC1/AgB1/H-11 (Major Histocompatibility Complex1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
1.13 pg/mL
Detection range:
1.88-120 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat MHCⅠ/AgBⅠ/H-1Ⅰ. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MHCⅠ/AgBⅠ/H-1Ⅰ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MHCⅠ/AgBⅠ/H-1Ⅰ, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat MHCⅠ/AgBⅠ/H-1Ⅰ. You can calculate the concentration of Rat MHCⅠ/AgBⅠ/H-1Ⅰ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-114
87-101
95-111
Average (%)
104
94
103
1:4
Range (%)
88-103
97-115
101-119
Average (%)
94
105
109
1:8
Range (%)
92-106
93-104
91-108
Average (%)
97
98
98
1:16
Range (%)
94-107
92-103
95-108
Average (%)
101
97
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-97
91
EDTA plasma (n=5)
99-116
107
Cell culture media (n=5)
91-107
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.79
12.53
52.32
5.37
11.77
52.63
Standard deviation
0.6
1.14
5.01
0.55
0.96
5.77
C V (%)
10.36
9.1
9.58
10.24
8.16
10.96
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat MHCⅠ/AgBⅠ/H-1Ⅰ concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MHCⅠ/AgBⅠ/H-1Ⅰ in samples. No significant cross-reactivity or interference between Rat MHCⅠ/AgBⅠ/H-1Ⅰ and analogues was observed.
