The Rat LEPR (Leptin Receptor) CLIA Kit is specifically designed for the quantitative measurement of leptin receptor levels in rat serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and precision, delivering accurate and consistent results for various research studies.The leptin receptor, also known as LEPR, is a key protein involved in regulating energy balance and metabolism.
Dysregulation of leptin signaling is linked to obesity, diabetes, and other metabolic disorders, highlighting the importance of studying LEPR levels in experimental models. The Rat LEPR CLIA Kit provides researchers with a valuable tool to explore the role of leptin receptor in metabolic pathways and potential therapeutic interventions.
Product Name:
Rat LEPR (Leptin Receptor) CLIA Kit
SKU:
RTES00605
Target:
Rat LEPR (Leptin Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat LEPR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat LEPR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat LEPR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat LEPR. You can calculate the concentration of Rat LEPR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-114
87-101
86-101
Average (%)
107
93
93
1:4
Range (%)
103-119
85-97
90-102
Average (%)
110
92
95
1:8
Range (%)
90-104
85-97
96-112
Average (%)
95
92
103
1:16
Range (%)
96-112
100-115
89-101
Average (%)
103
108
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
98
EDTA plasma (n=5)
84-97
91
Cell culture media (n=5)
89-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
36.78
78.59
296.14
39.21
78.46
286.07
Standard deviation
4.68
7.76
29.79
3.18
7.01
20.31
C V (%)
12.72
9.87
10.06
8.11
8.93
7.1
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat LEPR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat LEPR in samples. No significant cross-reactivity or interference between Rat LEPR and analogues was observed.
