The Rat LDL (Low-Density Lipoprotein) CLIA Kit is specifically designed for the precise measurement of LDL levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, providing consistent and accurate results for a variety of research purposes.LDL, also known as "bad" cholesterol, is a key player in the development of cardiovascular diseases such as atherosclerosis.
Monitoring LDL levels is crucial for understanding the progression of these conditions and evaluating potential treatment options.With its reliable performance and easy-to-use format, the Rat LDL CLIA Kit is an essential tool for researchers studying lipid metabolism, cardiovascular health, and related diseases in rat models. Get your hands on this kit today and take your research to the next level.
Product Name:
Rat LDL (Low Density Lipoprotein) CLIA Kit
SKU:
RTES00356
Target:
Rat LDL (Low Density Lipoprotein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 ng/mL
Detection range:
31.25-2000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat LDL. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat LDL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat LDL, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat LDL. You can calculate the concentration of Rat LDL in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-108
88-102
101-116
Average (%)
102
95
107
1:4
Range (%)
85-97
92-109
93-104
Average (%)
91
100
99
1:8
Range (%)
83-98
96-111
89-103
Average (%)
90
104
94
1:16
Range (%)
88-103
103-120
93-105
Average (%)
95
110
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-112
102
EDTA plasma (n=5)
95-109
102
Cell culture media (n=5)
101-115
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
104.54
228.98
827.23
94.18
206.67
893.08
Standard deviation
9.86
20.2
78.09
8.99
18.56
79.84
C V (%)
9.43
8.82
9.44
9.55
8.98
8.94
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat LDL concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat LDL in samples. No significant cross-reactivity or interference between Rat LDL and analogues was observed.
