Rat LDHA Recombinant Protein (RPPB1895)
- SKU:
- RPPB1895
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Rat
- Target:
- LDHA
- Synonyms:
- L-lactate dehydrogenase A chain
- LDH-A
- LDH musclesubunit
- LDH-M
- Source:
- Sf9 Insect cells
- Uniprot:
- P04642
Description
| Product Name: | Rat LDHA Recombinant Protein |
| Product Code: | RPPB1895 |
| Size: | 10µg |
| Species: | Rat |
| Target: | LDHA |
| Synonyms: | L-lactate dehydrogenase A chain, LDH-A, LDH musclesubunit, LDH-M. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | LDHA protein solution (0.25mg/ml) containing PhosphateBuffered Saline (pH 7.4), 1mM DTT and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
| Purity: | Greaterthan 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MAALKDQLIV NLLKEEQVPQ NKITVVGVGA VGMACAISIL MKDLADELALVDVIEDKLKG EMMDLQHGSL FLKTPKIVSS KDYSVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFIIPNVVKYSPQ CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HPLSCHGWVLGEHGDSSVPV WSGVNVAGVS LKSLNPQLGT DADKEQWKDV HKQVVDSAYE VIKLKGYTSW AIGLSVADLAESIMKNLRRV HPISTMIKGL YGIKEDVFLS VPCILGQNGI SDVVKVTLTP DEEARLKKSA DTLWGIQKEL QFLEHHHHHH |
LDHA catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. LDHA is localized primarily in muscle tissue and is part of the lactate dehydrogenase family. Mutations in LDHA have been linked to exertional myoglobinuria. LDH1 is decreased in essential thrombocythemia. LDHA is induced through a non-genomic pathway of estrogen action. Reduction in LDH-A activity results in stimulation of mitochondrial respiration and decrease of mitochondrial membrane potential.
LDHA Rat Recombinant produced in Sf9 Baculovirus cells isa single, glycosylated polypeptide chain containing 340 amino acids (1-332 a.a)and having a molecular mass of 37.5kDa (Migrates at 28-40kDa on SDS-PAGE underreducing conditions). LDHA is fused to an 8 amino acid His-tag at C-terminus& purified by proprietary chromatographic techniques.
| NCBI Summary: | mRNA expression in fibroblasts increases in response to induction by epidermal growth factor or serum [RGD, Feb 2006] |
| UniProt Code: | P04642 |
| NCBI GenInfo Identifier: | 8393706 |
| NCBI Gene ID: | 24533 |
| NCBI Accession: | NP_058721 |
| UniProt Related Accession: | P04642 |
| Molecular Weight: | 37.5kDa (340aa) 28-40KDa (SDS-PAGE under reducing conditions) |
| NCBI Full Name: | L-lactate dehydrogenase A chain |
| NCBI Synonym Full Names: | lactate dehydrogenase A |
| NCBI Official Symbol: | Ldha |
| NCBI Official Synonym Symbols: | CDK1; Ldh1 |
| NCBI Protein Information: | L-lactate dehydrogenase A chain |
| UniProt Protein Name: | L-lactate dehydrogenase A chain |
| UniProt Synonym Protein Names: | LDH muscle subunit; LDH-M |
| Protein Family: | L-lactate dehydrogenase |
| UniProt Gene Name: | Ldha |
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