The Rat LDHA (Lactate Dehydrogenase A) ELISA Kit is specifically designed for the accurate measurement of LDHA levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research applications.LDHA is a key enzyme involved in the conversion of lactate to pyruvate, playing a crucial role in energy metabolism and cellular respiration. Dysregulation of LDHA has been implicated in various diseases such as cancer, cardiovascular disorders, and metabolic diseases, making it a valuable biomarker for studying these conditions and evaluating potential therapeutic interventions.
With its user-friendly protocol and reliable performance, the Rat LDHA ELISA Kit is an essential tool for researchers studying LDHA function and its implications in disease pathology. Get accurate and reproducible results with this comprehensive and versatile assay kit.
Product Name:
Rat LDHA (Lactate Dehydrogenase A) ELISA Kit
SKU:
RTES01158
Target:
Rat LDHA (Lactate Dehydrogenase A)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat LDHA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat LDHA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat LDHA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat LDHA. You can calculate the concentration of Rat LDHA in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-110
89-101
90-106
Average (%)
105
96
97
1:4
Range (%)
90-102
85-96
88-101
Average (%)
95
90
94
1:8
Range (%)
91-102
80-92
84-100
Average (%)
96
87
91
1:16
Range (%)
87-102
83-96
88-102
Average (%)
93
88
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-99
92
EDTA plasma (n=5)
95-107
101
Cell culture media (n=5)
89-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.99
2.07
9.25
0.92
2.07
8.77
Standard deviation
0.07
0.1
0.4
0.06
0.1
0.31
C V (%)
7.07
4.83
4.32
6.52
4.83
3.53
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat LDHA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat LDHA in samples. No significant cross-reactivity or interference between Rat LDHA and analogues was observed.
