The Rat KIM-1 (Kidney Injury Molecule-1) CLIA Kit is a highly sensitive and specific assay designed for the quantitative detection of KIM-1 levels in rat serum, plasma, and urine samples. This kit provides accurate and reproducible results, making it an ideal tool for researchers studying kidney injury and related conditions.KIM-1 is a reliable biomarker for renal tubular injury, commonly associated with acute kidney injury and chronic kidney disease.
Monitoring KIM-1 levels can help in the early detection and monitoring of kidney damage, allowing for timely intervention and treatment. This CLIA kit offers a quick and easy way to measure KIM-1 levels in rat samples, providing valuable insights into renal health and function.
Product Name:
Rat KIM-1 (Kidney Injury Molecule 1) CLIA Kit
SKU:
RTES00353
Target:
Rat KIM-1 (Kidney Injury Molecule 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat KIM-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat KIM-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat KIM-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat KIM-1. You can calculate the concentration of Rat KIM-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-104
85-99
92-103
Average (%)
96
91
97
1:4
Range (%)
96-109
100-115
90-103
Average (%)
104
106
96
1:8
Range (%)
99-114
91-106
96-114
Average (%)
107
97
104
1:16
Range (%)
94-110
98-113
85-99
Average (%)
100
106
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-114
105
EDTA plasma (n=5)
93-111
101
Cell culture media (n=5)
97-110
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
52.01
126.84
426.41
54.76
120.47
439.89
Standard deviation
6.44
11.59
28.06
4.53
8.79
44.82
C V (%)
12.38
9.14
6.58
8.27
7.3
10.19
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat KIM-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat KIM-1 in samples. No significant cross-reactivity or interference between Rat KIM-1 and analogues was observed.
