Rat Interleukin 18 Binding Protein (IL-18BP) ELISA Kit

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SKU:
RTDL00549
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
F7F5P4
Sensitivity:
0.215ng/mL
Range:
0.625-40ng/mL
ELISA Type:
Sandwich
Synonyms:
IL18BPa,MC51L-53L-54L Homolog Gene Product,Tadekinig-alfa
Reactivity:
Rat
Sample Type:
Serum, Plasma And Other Biological Fluids
Frequently bought together:

Description

system_update_altDatasheet

Rat Interleukin 18 Binding Protein (IL-18BP) ELISA Kit

The Rat Interleukin-18 Binding Protein (IL18BP) ELISA Kit is specifically designed to accurately measure levels of IL18BP in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and consistent results for various research applications.IL18BP is a key regulator of the immune system, binding to and neutralizing interleukin-18 (IL-18) to prevent excessive inflammatory responses.

Abnormal levels of IL18BP have been associated with various inflammatory diseases and autoimmune disorders, highlighting its importance as a potential biomarker for studying and developing treatments for these conditions.Overall, the Rat IL18BP ELISA Kit provides researchers with a valuable tool for investigating the role of IL18BP in immune responses and inflammatory diseases in rat models.

Product Name:Rat Interleukin 18 Binding Protein (IL-18BP) ELISA Kit
Product Code:RTDL00549
Size:96 Assays
Target:IL18BP
Synonyms:IL18BPa, MC51L-53L-54L Homolog Gene Product, Tadekinig-alfa
Detection Method:Sandwich
Range:0.625-40ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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