Rat Interleukin 10 Receptor Alpha (IL-10Ra) ELISA Kit

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SKU:
RTDL00537
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
G3V830
Sensitivity:
0.063ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CDW210A,CD210A,IL10R-A,IL10-RA,CD210-A,HIL-10R,IL-10R1,IL10R,Cluster Of Differentiation W210A
Reactivity:
Rat
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
Frequently bought together:

Description

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Rat Interleukin 10 Receptor Alpha (IL-10Ra) ELISA Kit

The Rat Interleukin-10 Receptor Alpha (IL10RA) ELISA Kit is a reliable and accurate tool for measuring levels of IL10RA in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications.IL10RA is a key receptor involved in the anti-inflammatory response, playing a crucial role in immune regulation and tolerance.

Dysregulation of IL10RA has been implicated in various autoimmune and inflammatory diseases, making it a valuable biomarker for studying these conditions and developing targeted therapies.Overall, the Rat IL10RA ELISA Kit provides researchers with a powerful tool for investigating the role of IL10RA in health and disease, offering valuable insights into potential therapeutic interventions.

Product Name:Rat Interleukin 10 Receptor Alpha (IL-10Ra) ELISA Kit
Product Code:RTDL00537
Size:96 Assays
Target:IL10Ra
Synonyms:CDW210A, CD210A, IL10R-A, IL10-RA, CD210-A, HIL-10R, IL-10R1, IL10R, Cluster Of Differentiation W210A
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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