Rat Interferon Gamma Induced Protein 10kDa (IP10) ELISA Kit

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SKU:
RTDL00582
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P48973
Sensitivity:
11.5pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
CXCL10,C7,IFI10,INP10,SCYB10,Crg2,GIP10,Mob1,Chemokine C-X-C Motif Ligand 10,Small Inducible Cytokine Subfamily B(Cys-X-Cys)Member 10
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
Frequently bought together:

Description

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Rat Interferon Gamma Induced Protein 10kDa (IP10) ELISA Kit

The Rat Interferon-Gamma Induced Protein 10kDa (IP-10) ELISA Kit is specifically designed for the precise measurement of IP-10 levels in rat serum, plasma, and tissue lysates. With its exceptional sensitivity and specificity, this kit ensures accurate and reproducible results, making it an indispensable tool for various research applications.IP-10 is a key chemokine involved in immune responses, attracting and activating immune cells such as T cells and macrophages.

Its dysregulation has been implicated in various inflammatory and autoimmune diseases, underscoring its significance as a biomarker for studying these conditions and potential therapeutic interventions.With the Rat Interferon-Gamma Induced Protein 10kDa (IP-10) ELISA Kit, researchers can explore the role of IP-10 in immune regulation and inflammation, advancing our understanding of these complex processes and paving the way for novel treatment strategies.

Product Name:Rat Interferon Gamma Induced Protein 10kDa (IP10) ELISA Kit
Product Code:RTDL00582
Size:96 Assays
Target:IP10
Synonyms:CXCL10, C7, IFI10, INP10, SCYB10, Crg2, GIP10, Mob1, Chemokine C-X-C Motif Ligand 10, Small Inducible Cytokine Subfamily B(Cys-X-Cys)Member 10
Detection Method:Sandwich
Range:31.25-2000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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