The Rat INSR (Insulin Receptor) ELISA Kit is a powerful tool for measuring the levels of insulin receptor in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing precise and consistent results for various research purposes.The insulin receptor is a key component in regulating glucose uptake and metabolism in the body. Dysregulation of this receptor has been linked to diabetes, metabolic disorders, and other health conditions.
By accurately measuring insulin receptor levels, researchers can gain valuable insights into these diseases and potentially develop new therapeutic strategies.With its advanced technology and reliable performance, the Rat INSR ELISA Kit is a valuable asset for any laboratory studying insulin signaling pathways and related diseases in rats. Trust in this kit to provide accurate and insightful data for your research needs.
Product Name:
Rat INSR (Insulin Receptor) ELISA Kit
SKU:
RTES00923
Target:
Rat INSR (Insulin Receptor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat INSR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat INSR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat INSR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat INSR. You can calculate the concentration of Rat INSR in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
86-100
94-110
Average (%)
92
91
102
1:4
Range (%)
92-107
79-91
82-93
Average (%)
97
85
88
1:8
Range (%)
93-104
81-95
86-99
Average (%)
99
87
93
1:16
Range (%)
92-108
82-95
88-102
Average (%)
99
87
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
93
EDTA plasma (n=5)
92-103
98
Cell culture media (n=5)
94-107
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.05
2.96
9.73
1.03
3.0
8.87
Standard deviation
0.06
0.17
0.53
0.06
0.12
0.34
C V (%)
5.71
5.74
5.45
5.83
4.0
3.83
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat INSR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat INSR in samples. No significant cross-reactivity or interference between Rat INSR and analogues was observed.
