The Rat Insulin CLIA Kit is a highly sensitive and specific assay designed for the quantitative measurement of insulin levels in rat serum, plasma, and other biological samples. This kit offers reliable and reproducible results, making it an essential tool for researchers studying diabetes, metabolic disorders, and insulin resistance.Insulin is a key hormone that regulates blood sugar levels and plays a critical role in metabolism. Abnormal insulin levels have been linked to various health conditions such as type 2 diabetes, obesity, and cardiovascular diseases.
By accurately measuring insulin levels in rats, researchers can gain valuable insights into the mechanisms underlying these diseases and develop potential therapeutic strategies.Overall, the Rat Insulin CLIA Kit is an invaluable tool for researchers looking to study insulin physiology, metabolic disorders, and potential treatment interventions in rat models.
Product Name:
Rat INS (Insulin) CLIA Kit
SKU:
RTES00019
Target:
Rat INS (Insulin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat INS. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat INS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat INS, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat INS. You can calculate the concentration of Rat INS in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-106
88-98
89-101
Average (%)
98
93
96
1:4
Range (%)
102-120
93-105
93-108
Average (%)
110
98
99
1:8
Range (%)
87-98
89-101
98-114
Average (%)
92
94
105
1:16
Range (%)
95-107
91-106
104-117
Average (%)
100
97
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
94
EDTA plasma (n=5)
94-109
102
Cell culture media (n=5)
101-115
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.96
4.9
16.39
2.02
4.44
17.57
Standard deviation
0.25
0.53
1.26
0.17
0.52
1.84
C V (%)
12.76
10.82
7.69
8.42
11.71
10.47
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat INS concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat INS in samples. No significant cross-reactivity or interference between Rat INS and analogues was observed.
