The Rat IL3 (Interleukin-3) CLIA Kit is a highly sensitive and specific assay designed for the quantitative measurement of IL3 levels in rat samples such as serum, plasma, and tissue homogenates. This kit offers reliable and reproducible results, making it a valuable tool for researchers in various fields. Interleukin-3 is a cytokine that plays a critical role in the regulation of hematopoiesis and immune response. It is involved in the differentiation and proliferation of various immune cells, making it a key player in the immune system's function.
Abnormalities in IL3 levels have been linked to various diseases such as leukemia and autoimmune disorders, highlighting its importance as a potential biomarker for studying these conditions. With its easy-to-use format and accurate results, the Rat IL3 CLIA Kit is an essential tool for researchers studying the role of IL3 in disease pathogenesis and therapeutic development. Order now and take your research to the next level with this advanced assay kit.
Product Name:
Rat IL-3 CLIA Kit
SKU:
RTES00336
Target:
Rat IL-3
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IL-3. You can calculate the concentration of Rat IL-3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
95-108
91-102
Average (%)
99
101
97
1:4
Range (%)
104-118
97-115
96-109
Average (%)
110
105
104
1:8
Range (%)
83-95
85-98
90-106
Average (%)
90
91
97
1:16
Range (%)
100-117
85-101
98-111
Average (%)
108
92
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-111
102
EDTA plasma (n=5)
97-114
104
Cell culture media (n=5)
100-112
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.6
74.62
180.8
25.12
67.21
169.58
Standard deviation
2.79
6.61
15.84
2.36
6.77
12.33
C V (%)
11.34
8.86
8.76
9.39
10.07
7.27
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IL-3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IL-3 in samples. No significant cross-reactivity or interference between Rat IL-3 and analogues was observed.
