Interleukin 1 receptor, type II (IL1R2) also known as CD121b (Cluster of Differentiation 121b) is a cytokine receptor that belongs to the interleukin-1 receptor family. This protein binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I (IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. The pleiotropic cytokine IL1 is produced to regulate development and maintenance of the inflammatory responses, and binds to specific plasma membrane receptors on cells. Two distinct types of IL1 receptors which are able to bind IL1 specifically have been identified, designated as IL1RI (IL1RA) and IL1RII (IL1RB). IL1R1 contributes to IL-1 signaling, whereas the IL-1R2/CD121b has no signaling property and acts as a decoy for IL-1. IL-1R2/CD121b structurally consisting of a ligand binding portion comprised of three Ig-like domains, a single transmembrane region, and a short cytoplasmic domain, is expressed in a variety of cell types including B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Interleukin 4 (IL4) is reported to antagonize the activity of interleukin 1 by inducing the expression and release of this cytokine.
Product Name:
Rat IL1R2/CD121b Recombinant Protein (RPES0562)
Product Code:
RPES0562
Size:
50µg
Species:
Rat
Expressed Host:
HEK293 Cells
Synonyms:
IL1R2
Accession:
P43303
Sequence:
Met 1-Glu 355
Fusion tag:
C-His
Activity:
Measured by its ability to bind biotinylated Rat IL1B-His in a functional ELISA.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method
Protein Construction:
A DNA sequence encoding the rat IL1R2 (P43303) extracellular domain (Met 1-Glu 355) was expressed, fused with a polyhistidine tag at the C-terminus.
Purity:
> 97 % as determined by SDS-PAGE
Mol Mass:
39.7 kDa
AP Mol Mass:
55-60 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.5
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
