The Rat IL1R1 ELISA Kit (RTES01065) from Assay Genie is a powerful tool for accurately measuring levels of IL1R1 in rat samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reliable results, making it suitable for a diverse range of research applications.IL1R1, also known as Interleukin-1 receptor type 1, plays a critical role in the immune response and inflammation. By detecting IL1R1 levels, researchers can gain valuable insights into the mechanisms underlying various diseases, including autoimmune disorders, inflammatory conditions, and infectious diseases.
This kit is an essential resource for studying the role of IL1R1 in health and disease, advancing our understanding of immune regulation and potential therapeutic targets. Get your Rat IL1R1 ELISA Kit (RTES01065) from Assay Genie today and unlock new possibilities in your research.
Product Name:
Rat IL-1R1 ELISA Kit
SKU:
RTES01065
Target:
Rat IL-1R1
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-1R1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-1R1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-1R1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IL-1R1. You can calculate the concentration of Rat IL-1R1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-100
91-105
87-102
Average (%)
91
96
93
1:4
Range (%)
91-105
81-93
83-95
Average (%)
99
86
88
1:8
Range (%)
94-106
84-97
84-99
Average (%)
99
90
91
1:16
Range (%)
89-102
80-93
84-98
Average (%)
96
87
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
102
EDTA plasma (n=5)
91-106
98
Cell culture media (n=5)
92-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
243.4
691.14
2333.41
234.33
715.02
2327.87
Standard deviation
13.09
39.26
101.5
16.0
34.61
111.74
C V (%)
5.38
5.68
4.35
6.83
4.84
4.8
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat IL-1R1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IL-1R1 in samples. No significant cross-reactivity or interference between Rat IL-1R1 and analogues was observed.
