Interleukin-17 receptor (IL-17R), also known as Interleukin-17 receptor A (IL-17RA) and CD217 antigen (CD217), is a cytokine receptor which binds interleukin 17. IL-17R/IL-17RA (CD217) is a proinflammatory cytokine secreted by activated T-lymphocytes. It is a potent inducer of the maturation of CD34-positive hematopoietic precursors into neutrophils. IL-17R/IL-17RA (CD217) is a ubiquitous type I membrane glycoprotein that binds with low affinity to interleukin 17A. Interleukin 17A and its receptor IL-17RA play a pathogenic role in many inflammatory and autoimmune diseases such as rheumatoid arthritis. Like other cytokine receptors, this receptor likely has a multimeric structure. Defects in IL-17R/IL-17RA (CD217) are the cause of familial candidiasis type 5 (CANDF5). CANDF5 is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans.
Product Name:
Rat IL17RA Recombinant Protein (RPES0640)
Product Code:
RPES0640
Size:
100µg
Species:
Rat
Expressed Host:
HEK293 Cells
Synonyms:
IL17RA
Accession:
NP_001101353.2
Sequence:
Met1-Pro320
Fusion tag:
C-His
Activity:
1. Measured by its binding ability in a functional ELISA. Immobilized rat IL17RA-His at 10 µg/ml (100 ?l/well) can bind biotinylated human IL17A, The EC50 of biotinylated human IL17A is 5-11.8 ng/ml.2.Measured by its binding ability in a functional ELISA. Immobilized mouse IL17a at 10 µg/ml (100 ?l/well) can bind rat IL17RA-His, The EC50 of rat IL17RA-His is 0.44-1.02 µg/mL.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method
Protein Construction:
A DNA sequence encoding the rat IL17RA (NP_001101353.2) (Met1-Pro320) was expressed, fused with a polyhistidine tag at the C-terminus.
Purity:
> 95 % as determined by SDS-PAGE
Mol Mass:
34.1 kDa
AP Mol Mass:
52-58 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
