The Rat IL-16 CLIA Kit is a cutting-edge assay designed for the precise measurement of IL-16 levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.IL-16 is a key cytokine that plays a vital role in immune responses, particularly in inflammatory and autoimmune diseases. By quantifying IL-16 levels, researchers can gain valuable insights into the pathogenesis of these conditions and explore potential therapeutic interventions.
Whether studying immunology, inflammation, or disease mechanisms, the Rat IL-16 CLIA Kit provides a reliable tool for researchers seeking to advance their understanding of IL-16 biology and its implications in various health conditions. Trust in this kit to deliver precise and dependable results for your research needs.
Product Name:
Rat IL-16 CLIA Kit
SKU:
RTES00343
Target:
Rat IL-16
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-16. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-16 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-16, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IL-16. You can calculate the concentration of Rat IL-16 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
92-104
89-101
Average (%)
101
98
94
1:4
Range (%)
88-102
96-107
89-101
Average (%)
96
102
96
1:8
Range (%)
97-111
97-113
100-115
Average (%)
102
105
106
1:16
Range (%)
95-106
89-100
84-97
Average (%)
101
94
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-107
99
EDTA plasma (n=5)
88-101
93
Cell culture media (n=5)
94-111
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.1
52.11
188.1
28.35
53.03
187.54
Standard deviation
3.14
4.52
15.31
2.94
6.33
15.02
C V (%)
11.59
8.67
8.14
10.37
11.94
8.01
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IL-16 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IL-16 in samples. No significant cross-reactivity or interference between Rat IL-16 and analogues was observed.
