The Rat IL12 p40 CLIA Kit is a powerful tool for researchers looking to accurately measure levels of interleukin 12 (IL-12) p40 in rat samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing reliable and reproducible results for a variety of research applications.IL-12 p40 is a subunit of the IL-12 cytokine, which plays a critical role in regulating the immune response by promoting the differentiation of T cells and the production of interferon-gamma. Dysregulation of IL-12 signaling has been implicated in various inflammatory and autoimmune diseases, making it a key target for drug development and therapeutic interventions.
By using the Rat IL12 p40 CLIA Kit, researchers can gain valuable insights into the role of IL-12 in immune responses, inflammation, and disease pathogenesis. This kit is a valuable tool for studying the complex interplay between the immune system and various disease states in rat models, ultimately leading to a better understanding of disease mechanisms and potential therapeutic strategies.
Product Name:
Rat IL-12/p40 CLIA Kit
SKU:
RTES00040
Target:
Rat IL-12/p40
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
1.88 pg/mL
Detection range:
3.13-200 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-12 p40. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-12 p40 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-12 p40, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IL-12 p40. You can calculate the concentration of Rat IL-12 p40 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-118
93-109
104-119
Average (%)
110
100
110
1:4
Range (%)
85-100
91-106
88-98
Average (%)
91
97
93
1:8
Range (%)
89-102
87-100
97-109
Average (%)
95
92
102
1:16
Range (%)
89-101
97-111
97-110
Average (%)
96
104
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-108
99
EDTA plasma (n=5)
87-102
93
Cell culture media (n=5)
93-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.2
29.41
83.88
9.05
31.63
88.96
Standard deviation
0.97
2.62
6.5
0.86
2.49
8.49
C V (%)
10.54
8.91
7.75
9.5
7.87
9.54
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IL-12 p40 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IL-12 p40 in samples. No significant cross-reactivity or interference between Rat IL-12 p40 and analogues was observed.
