Rat IGFBP-2 (Insulin-Like Growth Factor Binding Protein 2) CLIA Kit
The Rat IGFBP-2 (Insulin-like Growth Factor Binding Protein 2) CLIA Kit is a powerful tool for accurately measuring IGFBP-2 levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and repeatable results for a variety of research applications.IGFBP-2 is a key regulator of insulin-like growth factor signaling pathways, playing a critical role in cell growth, differentiation, and development. Dysregulation of IGFBP-2 has been linked to various diseases, including cancer, diabetes, and metabolic disorders, making it a valuable biomarker for understanding these conditions and exploring potential therapeutic interventions.
With the Rat IGFBP-2 CLIA Kit, researchers can delve deeper into the intricate mechanisms of IGFBP-2 function and its implications in disease pathology, paving the way for innovative research discoveries and personalized medicine strategies.
Product Name:
Rat IGFBP-2 (Insulin-Like Growth Factor Binding Protein 2) CLIA Kit
SKU:
RTES00317
Target:
Rat IGFBP-2 (Insulin-Like Growth Factor Binding Protein 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IGFBP-2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IGFBP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IGFBP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IGFBP-2. You can calculate the concentration of Rat IGFBP-2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-108
87-100
86-99
Average (%)
99
92
93
1:4
Range (%)
99-113
92-106
90-107
Average (%)
104
97
97
1:8
Range (%)
89-102
87-100
103-118
Average (%)
94
93
109
1:16
Range (%)
91-103
89-104
90-102
Average (%)
97
95
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-113
103
EDTA plasma (n=5)
94-110
100
Cell culture media (n=5)
97-110
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
96.98
211.47
995.76
90.34
197.23
988.13
Standard deviation
8.91
15.9
82.75
7.5
16.78
78.16
C V (%)
9.19
7.52
8.31
8.3
8.51
7.91
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IGFBP-2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IGFBP-2 in samples. No significant cross-reactivity or interference between Rat IGFBP-2 and analogues was observed.
