The Rat IGF-2 (Insulin-like Growth Factor-2) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of IGF-2 levels in rat serum, plasma, and tissue homogenates. This kit offers reliable and reproducible results, making it ideal for various research applications in the fields of endocrinology, oncology, and developmental biology.IGF-2 is a key growth factor involved in regulating cell growth, differentiation, and metabolism. It plays a crucial role in fetal development, tissue repair, and the regulation of insulin sensitivity.
Abnormal levels of IGF-2 have been implicated in various diseases, including cancer, diabetes, and metabolic disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions. The Rat IGF-2 CLIA Kit from Assaygenie provides researchers with a powerful tool for studying the role of IGF-2 in health and disease, with high sensitivity and specificity to ensure accurate and reliable results.
Product Name:
Rat IGF-2 (Insulin Like Growth Factor 2) CLIA Kit
SKU:
RTES00315
Target:
Rat IGF-2 (Insulin Like Growth Factor 2)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IGF-2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IGF-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IGF-2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IGF-2. You can calculate the concentration of Rat IGF-2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-115
102-115
102-117
Average (%)
106
109
109
1:4
Range (%)
90-103
92-105
90-106
Average (%)
95
98
97
1:8
Range (%)
95-108
89-101
96-110
Average (%)
101
94
104
1:16
Range (%)
98-114
98-115
88-101
Average (%)
104
106
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
92
EDTA plasma (n=5)
94-109
99
Cell culture media (n=5)
86-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
37.42
76.07
304.23
38.03
81.89
286.69
Standard deviation
3.93
6.21
30.97
3.44
9.71
20.73
C V (%)
10.5
8.16
10.18
9.05
11.86
7.23
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IGF-2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IGF-2 in samples. No significant cross-reactivity or interference between Rat IGF-2 and analogues was observed.
