The Rat IgE (Immunoglobulin E) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of IgE levels in rat serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it an ideal tool for a variety of research applications.Immunoglobulin E plays a crucial role in allergic reactions and immune responses in rats. Elevated levels of IgE have been linked to various allergic conditions, making it an important biomarker for studying these diseases and developing potential treatments.
The Rat IgE CLIA Kit is easy to use and provides quick results, allowing researchers to efficiently study the role of IgE in allergic responses and immune disorders in rats. This kit is a valuable asset for any laboratory conducting research in the field of immunology and allergy.
Product Name:
Rat IgE (Immunoglobulin E) CLIA Kit
SKU:
RTES00304
Target:
Rat IgE (Immunoglobulin E)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IgE. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IgE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IgE, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IgE. You can calculate the concentration of Rat IgE in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-115
101-120
98-112
Average (%)
108
109
106
1:4
Range (%)
98-111
102-119
100-115
Average (%)
103
109
109
1:8
Range (%)
90-103
92-103
86-98
Average (%)
96
98
91
1:16
Range (%)
101-117
88-100
95-107
Average (%)
109
95
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-100
95
EDTA plasma (n=5)
90-103
96
Cell culture media (n=5)
90-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.06
1.76
7.68
0.97
1.93
8.24
Standard deviation
0.09
0.15
0.84
0.08
0.19
0.52
C V (%)
8.49
8.52
10.94
8.25
9.84
6.31
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IgE concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IgE in samples. No significant cross-reactivity or interference between Rat IgE and analogues was observed.
