Rat Hypoxia Inducible Factor 1 Alpha (HIF1a) ELISA Kit
SKU:
RTDL00485
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O35800
Sensitivity:
0.057ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
HIF1-A,MOP1,PASD8,Basic Helix-Loop-Helix Transcription Factor,ARNT-interacting protein,Basic-helix-loop-helix-PAS protein MOP1,Class E basic helix-loop-helix protein 78
Reactivity:
Rat
Sample Type:
Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
Rat Hypoxia Inducible Factor 1 Alpha (HIF1a) ELISA Kit
The Rat Hypoxia-Inducible Factor 1 Alpha (HIF1A) ELISA Kit is a powerful tool for accurate measurement of HIF1A levels in rat samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and reproducible results, making it ideal for a variety of research applications.HIF1A is a key transcription factor that plays a critical role in cellular adaptation to hypoxia, regulating the expression of genes involved in metabolism, angiogenesis, and cell survival.
Dysregulation of HIF1A has been implicated in various diseases including cancer, ischemic diseases, and inflammatory disorders, making it a valuable biomarker for understanding disease mechanisms and identifying potential therapeutic targets.Overall, the Rat HIF1A ELISA Kit from Assaygenie provides researchers with a valuable tool for studying the role of HIF1A in various physiological and pathological processes, paving the way for future advancements in biomedical research and drug development.
Product Name:
Rat Hypoxia Inducible Factor 1 Alpha (HIF1a) ELISA Kit
Product Code:
RTDL00485
Size:
96 Assays
Target:
HIF1a
Synonyms:
HIF1-A, MOP1, PASD8, Basic Helix-Loop-Helix Transcription Factor, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
