The Rat HSP47 (Heat Shock Protein) CLIA Kit is specifically designed for the quantitative measurement of HSP47 levels in rat serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, guaranteeing precise and consistent results, making it a valuable tool for various research purposes.HSP47 is a key molecular chaperone that plays a critical role in protein folding and collagen biosynthesis.
Its dysregulation has been linked to various diseases, including fibrosis, cancer, and inflammatory disorders, making it a crucial biomarker for understanding and potentially treating these conditions.Overall, the Rat HSP47 CLIA Kit offers a reliable and efficient method for studying the role of HSP47 in disease pathogenesis and could potentially lead to the development of novel therapeutic strategies.
Product Name:
Rat HSP47 (Heat Shock Protein) CLIA Kit
SKU:
RTES00281
Target:
Rat HSP47 (Heat Shock Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat HSP47. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HSP47 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HSP47, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat HSP47. You can calculate the concentration of Rat HSP47 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-100
99-117
86-97
Average (%)
94
107
91
1:4
Range (%)
102-116
88-99
102-120
Average (%)
109
94
110
1:8
Range (%)
92-108
89-100
100-117
Average (%)
99
95
107
1:16
Range (%)
85-97
99-115
84-98
Average (%)
91
106
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-101
94
EDTA plasma (n=5)
88-100
94
Cell culture media (n=5)
92-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.92
2.99
7.03
0.87
2.75
7.45
Standard deviation
0.1
0.26
0.44
0.09
0.28
0.57
C V (%)
10.87
8.7
6.26
10.34
10.18
7.65
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat HSP47 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat HSP47 in samples. No significant cross-reactivity or interference between Rat HSP47 and analogues was observed.
