Rat HIF-1 alpha (Hypoxia Inducible Factor 1 Alpha) CLIA Kit
The Rat HIF-1 Alpha (Hypoxia Inducible Factor 1 Alpha) CLIA Kit is specifically designed for the quantitative detection of HIF-1 Alpha levels in rat samples. This kit offers high sensitivity and accuracy, providing reliable and consistent results for various research applications. HIF-1 Alpha is a key transcription factor that plays a crucial role in response to hypoxia, regulating the expression of genes involved in adaptation to low oxygen levels. Dysregulation of HIF-1 Alpha is associated with various diseases, including cancer, cardiovascular disorders, and ischemic conditions.
Therefore, monitoring HIF-1 Alpha levels can provide valuable insights into these pathologies and aid in the development of potential therapeutic interventions. Overall, the Rat HIF-1 Alpha CLIA Kit is a valuable tool for researchers studying hypoxia-related pathways and exploring the role of HIF-1 Alpha in various physiological and pathological conditions in rats.
Product Name:
Rat HIF-1 alpha (Hypoxia Inducible Factor 1 Alpha) CLIA Kit
SKU:
RTES00302
Target:
Rat HIF-1 alpha (Hypoxia Inducible Factor 1 Alpha)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
1.88 pg/mL
Detection range:
3.13-200 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat HIF-1α. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HIF-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HIF-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat HIF-1α. You can calculate the concentration of Rat HIF-1α in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-102
90-103
94-108
Average (%)
95
97
99
1:4
Range (%)
98-115
89-105
94-110
Average (%)
105
96
102
1:8
Range (%)
92-106
88-102
91-106
Average (%)
97
95
97
1:16
Range (%)
100-113
86-102
92-105
Average (%)
105
93
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-114
106
EDTA plasma (n=5)
93-109
100
Cell culture media (n=5)
88-100
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.04
25.66
75.85
9.46
24.99
75.05
Standard deviation
0.86
1.87
4.79
0.81
2.79
7.78
C V (%)
8.57
7.29
6.32
8.56
11.16
10.37
Sample type &Sample volume:
Tissue homogenates,cell lysates and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat HIF-1α concentrations in Tissue homogenates,cell lysates and other biological fluids.
Specificity:
This kit recognizes Rat HIF-1α in samples. No significant cross-reactivity or interference between Rat HIF-1α and analogues was observed.
