Epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein, and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
Product Name:
Rat HER2/ErbB2 Recombinant Protein (RPES0099)
Product Code:
RPES0099
Size:
20µg
Species:
Rat
Expressed Host:
HEK293 Cells
Synonyms:
ErbB2,Neu,p185erbB2
Accession:
AAH61863.1
Sequence:
Met 4-Thr 656
Activity:
1.Measured by its binding ability in a functional ELISA. 2.Immobilized rat ErbB2 at 10µg/mL (100?L/well) can bind herceptin, the EC50 of herceptin is 30-90ng/mL.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method
Protein Construction:
A DNA sequence encoding the rat ErbB2 (AAH61863.1) extracellular domain (Met 4-Thr 656) was expressed and purified, with seven additional amino acids (ALEVLFQ) at the C-terminus.
Purity:
> 92 % as determined by SDS-PAGE
Mol Mass:
70.6 kDa
AP Mol Mass:
95-105 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
