The Rat Heat Shock Protein Beta 8 (HSPB8) ELISA Kit is a powerful tool for accurately measuring levels of HSPB8 in rat samples such as serum, plasma, and tissue lysates. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research studies.HSPB8 is a vital heat shock protein that plays a key role in cellular stress response and protein folding. Dysregulation of HSPB8 has been linked to various diseases, including neurodegenerative disorders and muscle diseases.
By accurately measuring HSPB8 levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.Overall, the Rat Heat Shock Protein Beta 8 (HSPB8) ELISA Kit is a reliable tool for studying the role of HSPB8 in health and disease, offering researchers a comprehensive and efficient solution for their research needs.
Product Name:
Rat Heat Shock Protein Beta 8 (HSPb8) ELISA Kit
Product Code:
RTDL00512
Size:
96 Assays
Target:
HSPb8
Synonyms:
CMT2L, DHMN2, E2IG1, H11, HMN2, HSP22, Heat Shock 22kDa Protein 8, Alpha-crystallin C chain, E2-induced gene 1 protein, Small stress protein-like protein HSP22
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-99
91
EDTA plasma (n=5)
80-99
89
Heparin plasma (n=5)
82-99
90
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
