The Rat Heat Shock Protein Beta 6 (HSPB6) ELISA Kit is a powerful tool for the quantification of HSPB6 levels in rat samples, including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers precise and consistent results, making it an invaluable asset for various research purposes.HSPB6, also known as heat shock protein 20 (Hsp20), is a key player in cellular stress response, playing a crucial role in protecting cells from damage and maintaining protein homeostasis. Dysregulation of HSPB6 has been implicated in various pathological conditions, such as heart diseases, neurodegenerative disorders, and cancer, highlighting its importance as a potential biomarker and therapeutic target.
By using the Rat HSPB6 ELISA Kit, researchers can accurately measure HSPB6 levels in rat samples, providing valuable insights into its function and its involvement in disease pathogenesis. This kit offers a reliable and efficient solution for studying HSPB6 biology and exploring its potential implications in various disease contexts.
Product Name:
Rat Heat Shock Protein Beta 6 (HSPb6) ELISA Kit
Product Code:
RTDL00511
Size:
96 Assays
Target:
HSPb6
Synonyms:
HSP20, Heat Shock Protein 20 kD, Heat Shock Protein B6, Alpha Crystallin Related, Heat shock 20 kDa-like protein p20
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-99
91
EDTA plasma (n=5)
80-99
89
Heparin plasma (n=5)
82-99
90
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
