The Rat Heat Shock 70kDa Protein (HSPA5) ELISA Kit is specifically designed for the precise measurement of HSPA5 levels in rat serum, plasma, and cell culture supernatants. This kit provides exceptional sensitivity and specificity, ensuring consistent and accurate results for various research applications.HSPA5, also known as BiP or GRP78, is a vital protein involved in the cellular stress response and protein folding processes. It plays a crucial role in maintaining cell homeostasis and protecting cells from various stressors.
Dysregulation of HSPA5 expression has been linked to numerous diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases, making it a valuable biomarker for understanding these conditions and developing potential therapeutic interventions.With its reliable performance and ease of use, the Rat Heat Shock 70kDa Protein (HSPA5) ELISA Kit is an indispensable tool for researchers studying the role of HSPA5 in health and disease.
Product Name:
Rat Heat Shock 70kDa Protein 5 (HSPA5) ELISA Kit
Product Code:
RTDL00507
Size:
96 Assays
Target:
HSPA5
Synonyms:
MIF2, BIP, GRP78, Immunoglobulin heavy chain-binding protein, Glucose Regulated Protein 78, Binding Immunoglobulin Protein, Endoplasmic reticulum lumenal Ca binding grp78
Detection Method:
Sandwich
Range:
0.781-50ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
