Rat HDGF (Hepatoma Derived Growth Factor) CLIA Kit
The Rat HDGF (Hepatoma-Derived Growth Factor) CLIA Kit is a highly sensitive and specific assay designed for the detection of HDGF levels in rat samples. This kit is ideal for researchers studying the role of HDGF in various biological processes, including cell growth, differentiation, and development. HDGF is a growth factor that plays a critical role in promoting cell proliferation and survival, making it an important target for cancer research and therapy development. By accurately measuring HDGF levels in rat samples, researchers can gain valuable insights into the mechanisms underlying cancer progression and potentially identify new therapeutic targets.
The Rat HDGF CLIA Kit offers reliable and reproducible results, making it a valuable tool for studying the physiological and pathological functions of HDGF in rat models. With its high sensitivity and specificity, this kit is suitable for a wide range of research applications, providing researchers with valuable data for advancing their understanding of HDGF biology.
Product Name:
Rat HDGF (Hepatoma Derived Growth Factor) CLIA Kit
SKU:
RTES00294
Target:
Rat HDGF (Hepatoma Derived Growth Factor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat HDGF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HDGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HDGF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat HDGF. You can calculate the concentration of Rat HDGF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-107
89-103
100-116
Average (%)
100
94
106
1:4
Range (%)
101-119
101-118
90-101
Average (%)
110
108
95
1:8
Range (%)
86-101
103-118
98-109
Average (%)
93
110
104
1:16
Range (%)
87-98
86-97
89-100
Average (%)
93
92
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-107
99
EDTA plasma (n=5)
91-105
98
Cell culture media (n=5)
98-111
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
217.85
637.88
1675.71
219.88
649.98
1576.67
Standard deviation
20.35
64.81
179.64
20.16
76.24
154.2
C V (%)
9.34
10.16
10.72
9.17
11.73
9.78
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat HDGF concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat HDGF in samples. No significant cross-reactivity or interference between Rat HDGF and analogues was observed.
