Rat Growth Differentiation Factor 15 (GDF15) ELISA Kit

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SKU:
RTDL00422
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9Z0J6
Sensitivity:
5.6pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
PDF,MIC1,NAG-1,PLAB,PTGFB,Macrophage inhibitory cytokine 1,NSAID-activated gene 1 protein,Placental bone morphogenetic protein,Prostate differentiation factor
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
Frequently bought together:

Description

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Rat Growth Differentiation Factor 15 (GDF15) ELISA Kit

The Rat GDF15 (Growth Differentiation Factor 15) ELISA Kit is a powerful tool for the accurate measurement of GDF15 levels in rat serum, plasma, and tissue culture supernatants. This kit is known for its high sensitivity and specificity, providing researchers with reliable and consistent results for their studies.GDF15 is a protein that plays a critical role in regulating cell growth, differentiation, and inflammation.

It is involved in various biological processes, including cancer, obesity, and cardiovascular diseases, making it a valuable biomarker for understanding these conditions and developing targeted treatments.With the Rat GDF15 ELISA Kit, researchers can confidently explore the role of GDF15 in different physiological and pathological conditions, paving the way for new discoveries and potential therapeutic interventions.

Product Name:Rat Growth Differentiation Factor 15 (GDF15) ELISA Kit
Product Code:RTDL00422
Size:96 Assays
Target:GDF15
Synonyms:PDF, MIC1, NAG-1, PLAB, PTGFB, Macrophage inhibitory cytokine 1, NSAID-activated gene 1 protein, Placental bone morphogenetic protein, Prostate differentiation factor
Detection Method:Sandwich
Range:15.6-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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