Rat GROgamma (Growth Regulated Oncogene Gamma) CLIA Kit
The Rat GROgamma (Growth Regulated Oncogene gamma) CLIA Kit is a highly sensitive assay designed for the precise measurement of GROgamma levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional specificity and accuracy, ensuring consistent and trustworthy results for various research purposes.GROgamma is a key chemokine involved in inflammatory processes and plays a critical role in the regulation of immune responses. Elevated levels of GROgamma have been associated with various inflammatory conditions, making it an important biomarker for studying immune-related diseases and potential therapeutic interventions.
With its superior performance and reliability, the Rat GROgamma CLIA Kit is an indispensable tool for researchers studying inflammation, immune responses, and related diseases in rat models. Get accurate and reproducible results with this advanced assay kit from Assay Genie.
Product Name:
Rat GROgamma (Growth Regulated Oncogene Gamma) CLIA Kit
SKU:
RTES00419
Target:
Rat GROgamma (Growth Regulated Oncogene Gamma)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GROγ/CXCL3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GROγ/CXCL3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GROγ/CXCL3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GROγ/CXCL3. You can calculate the concentration of Rat GROγ/CXCL3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-117
103-119
86-98
Average (%)
109
110
93
1:4
Range (%)
89-101
88-101
99-116
Average (%)
94
94
107
1:8
Range (%)
89-104
95-107
94-108
Average (%)
95
100
101
1:16
Range (%)
95-109
92-108
103-119
Average (%)
102
99
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-114
108
EDTA plasma (n=5)
92-104
98
Cell culture media (n=5)
88-100
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.74
58.05
208.0
27.28
58.7
194.25
Standard deviation
2.35
4.68
19.39
2.8
5.01
13.09
C V (%)
8.79
8.06
9.32
10.26
8.53
6.74
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GROγ/CXCL3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GROγ/CXCL3 in samples. No significant cross-reactivity or interference between Rat GROγ/CXCL3 and analogues was observed.
