Rat GRO beta (Growth Regulated Oncogene Beta) CLIA Kit
The Rat GRO-beta (Growth Regulated Oncogene-beta) CLIA Kit is a highly reliable and sensitive assay designed for the accurate detection of GRO-beta levels in rat serum, plasma, and cell culture supernatants. This kit ensures precise and reproducible results, making it suitable for various research applications.GRO-beta, also known as CXCL2, is a key chemokine involved in inflammatory responses and immune cell recruitment. It is implicated in various pathological conditions, including cancer, inflammation, and autoimmune diseases.
The accurate measurement of GRO-beta levels is essential for studying these diseases and developing potential therapeutic interventions.Overall, the Rat GRO-beta CLIA Kit provides researchers with a powerful tool for investigating the role of GRO-beta in disease progression and exploring its potential as a biomarker for diagnostic and therapeutic purposes.
Product Name:
Rat GRO beta (Growth Regulated Oncogene Beta) CLIA Kit
SKU:
RTES00418
Target:
Rat GRO beta (Growth Regulated Oncogene Beta)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GROβ/CXCL2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GROβ/CXCL2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GROβ/CXCL2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GROβ/CXCL2. You can calculate the concentration of Rat GROβ/CXCL2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-113
103-116
97-114
Average (%)
106
109
104
1:4
Range (%)
101-118
94-109
101-113
Average (%)
109
99
107
1:8
Range (%)
96-111
99-111
85-97
Average (%)
102
105
92
1:16
Range (%)
90-102
91-105
89-104
Average (%)
96
96
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
100
EDTA plasma (n=5)
98-108
103
Cell culture media (n=5)
88-100
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
40.56
81.89
332.23
36.51
88.8
303.3
Standard deviation
4.19
6.64
34.02
3.27
9.45
30.0
C V (%)
10.33
8.11
10.24
8.96
10.64
9.89
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GROβ/CXCL2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GROβ/CXCL2 in samples. No significant cross-reactivity or interference between Rat GROβ/CXCL2 and analogues was observed.
