The Rat GR (Glucocorticoid Receptor) CLIA Kit is specifically designed for the quantitative determination of glucocorticoid receptor levels in rat samples, including serum, plasma, and tissue lysates. This kit offers superior sensitivity and specificity, providing accurate and reliable results for various research applications.The glucocorticoid receptor is a key regulator of various physiological processes and is involved in the response to stress, inflammation, and metabolism. Dysregulation of glucocorticoid receptor signaling is associated with various diseases, including autoimmune disorders, metabolic syndromes, and mental health conditions.
Therefore, the Rat GR CLIA Kit is a valuable tool for investigating the role of glucocorticoid receptors in disease pathogenesis and for developing potential therapeutic interventions.Overall, the Rat GR CLIA Kit offers researchers a robust and convenient solution for studying glucocorticoid receptor biology in rats, enabling in-depth exploration of its functional significance in health and disease.
Product Name:
Rat GR (Glucocorticoid Receptor) CLIA Kit
SKU:
RTES00239
Target:
Rat GR (Glucocorticoid Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GR. You can calculate the concentration of Rat GR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-108
91-107
99-116
Average (%)
99
97
106
1:4
Range (%)
91-106
89-103
89-103
Average (%)
99
94
97
1:8
Range (%)
91-104
99-114
91-103
Average (%)
98
104
98
1:16
Range (%)
95-106
98-111
87-98
Average (%)
100
105
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-105
96
EDTA plasma (n=5)
86-98
92
Cell culture media (n=5)
88-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
98.91
196.48
690.66
106.7
200.99
735.86
Standard deviation
9.06
20.96
77.84
10.26
15.72
75.13
C V (%)
9.16
10.67
11.27
9.62
7.82
10.21
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GR in samples. No significant cross-reactivity or interference between Rat GR and analogues was observed.
