Rat GR beta (Glucocorticoid Receptor beta) CLIA Kit
The Rat GR (Glucocorticoid Receptor) Beta CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of glucocorticoid receptor beta levels in rat samples. This kit is suitable for use with serum, plasma, or cell culture supernatants, providing reliable and reproducible results for a variety of research applications.The glucocorticoid receptor beta is a key protein involved in the regulation of various biological processes, including immune response, metabolism, and stress.
Dysregulation of this receptor has been linked to conditions such as inflammation, autoimmune diseases, and metabolic disorders, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.With its user-friendly format and high-quality components, the Rat GR Beta CLIA Kit from Assay Genie is an essential tool for researchers looking to accurately measure glucocorticoid receptor beta levels in rat samples.
Product Name:
Rat GR beta (Glucocorticoid Receptor beta) CLIA Kit
SKU:
RTES00241
Target:
Rat GR beta (Glucocorticoid Receptor beta)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GRβ. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GRβ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GRβ, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GRβ. You can calculate the concentration of Rat GRβ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-104
92-102
93-105
Average (%)
97
97
99
1:4
Range (%)
89-104
96-109
100-118
Average (%)
95
103
108
1:8
Range (%)
98-112
97-108
100-115
Average (%)
104
102
106
1:16
Range (%)
91-104
94-106
89-101
Average (%)
98
99
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-104
99
EDTA plasma (n=5)
93-109
100
Cell culture media (n=5)
90-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.29
74.49
221.97
23.67
69.17
222.86
Standard deviation
2.1
5.26
14.1
2.5
5.75
20.86
C V (%)
7.99
7.06
6.35
10.56
8.31
9.36
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GRβ concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GRβ in samples. No significant cross-reactivity or interference between Rat GRβ and analogues was observed.
