Rat Glutamate Cysteine Ligase, Catalytic (GCLC) ELISA Kit

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SKU:
RTDL00417
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P19468
Sensitivity:
0.33ng/mL
Range:
0.781-50ng/mL
ELISA Type:
Sandwich
Synonyms:
GCS,GLCL,GLCLC,Gamma-glutamylcysteine synthetase,Gamma-ECS
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
Frequently bought together:

Description

system_update_altDatasheet

Rat Glutamate Cysteine Ligase, Catalytic (GCLC) ELISA Kit

The Rat Glutamate-Cysteine Ligase Catalytic (GCLC) ELISA Kit is a powerful tool for analyzing levels of GCLC in rat samples, including serum, plasma, and tissue homogenates. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it perfect for a variety of research applications.GCLC is an essential enzyme involved in the synthesis of glutathione, a key antioxidant that plays a crucial role in protecting cells from oxidative stress and maintaining cellular homeostasis.

Dysregulation of GCLC has been linked to various diseases, including neurodegenerative disorders, liver diseases, and cancer. Therefore, the Rat GCLC ELISA Kit is an invaluable resource for studying the role of GCLC in these conditions and exploring potential therapeutic interventions.

Product Name:Rat Glutamate Cysteine Ligase, Catalytic (GCLC) ELISA Kit
Product Code:RTDL00417
Size:96 Assays
Target:GCLC
Synonyms:GCS, GLCL, GLCLC, Gamma-glutamylcysteine synthetase, Gamma-ECS
Detection Method:Sandwich
Range:0.78 -50ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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