SLC2A1,SLC2-A1,GLUT,Solute Carrier Family 2 Member 1,Facilitated Glucose Transporter,Glucose transporter type 1, erythrocyte/brain,HepG2 glucose transporter
The Rat Glucose Transporter 1 (GLUT1) ELISA Kit is specifically designed for the quantitative measurement of GLUT1 levels in rat samples. With its high sensitivity and specificity, this kit ensures accurate and reliable results for researchers studying glucose transport and metabolism in rats.GLUT1 is a key protein involved in glucose uptake and is essential for maintaining glucose homeostasis in the body. Dysregulation of GLUT1 has been linked to various metabolic disorders, including diabetes and cancer.
By accurately measuring GLUT1 levels in rat samples, researchers can gain valuable insights into the role of glucose transport in these diseases and develop potential therapeutic strategies.Don't miss out on the opportunity to advance your research with the Rat GLUT1 ELISA Kit. Order now and unlock valuable data on GLUT1 expression in rat samples for your studies.
Product Name:
Rat Glucose Transporter 1 (GLUT1) ELISA Kit
Product Code:
RTDL00440
Size:
96 Assays
Target:
GLUT1
Synonyms:
SLC2A1, SLC2-A1, GLUT, Solute Carrier Family 2 Member 1,Facilitated Glucose Transporter, Glucose transporter type 1, erythrocyte/brain, HepG2 glucose transporter
Detection Method:
Sandwich
Range:
1.25-80ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
