Rat Glucose-6-Phosphatase, Catalytic (G6PC) ELISA Kit

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SKU:
RTDL00401
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P43428
Sensitivity:
0.65ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
G6PT,GSD1a,Glycogen Storage Disease Type I,von Gierke Disease,Glucose-6-phosphatase alpha
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
Frequently bought together:

Description

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Rat Glucose-6-Phosphatase, Catalytic (G6PC) ELISA Kit

The Rat Glucose-6-Phosphatase Catalytic (G6PC) ELISA Kit is specifically designed for the accurate detection of G6PC levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a wide range of research applications.G6PC is a key enzyme involved in glucose homeostasis, playing a critical role in gluconeogenesis and glycogenolysis. Dysregulation of G6PC has been linked to metabolic disorders such as diabetes, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.

With easy-to-follow protocols and user-friendly features, the Rat G6PC ELISA Kit is a valuable tool for researchers studying metabolic disorders and investigating the role of G6PC in glucose metabolism. Order now to advance your research with confidence and precision.

Product Name:Rat Glucose-6-Phosphatase, Catalytic (G6PC) ELISA Kit
Product Code:RTDL00401
Size:96 Assays
Target:G6PC
Synonyms:G6PT, GSD1a, Glycogen Storage Disease Type I,von Gierke Disease, Glucose-6-phosphatase alpha
Detection Method:Sandwich
Range:1.56-100ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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