The Rat GLP-1 (Glucagon-Like Peptide 1) CLIA Kit is a cutting-edge tool for measuring GLP-1 levels in rat samples with high precision and accuracy. This kit is specially designed for detecting GLP-1 in serum, plasma, and cell culture supernatants, allowing researchers to obtain reliable and consistent results for their studies.GLP-1 is a key hormone that regulates glucose metabolism and insulin secretion, making it a critical target for diabetes research and therapeutic development.
By accurately quantifying GLP-1 levels, this kit enables researchers to gain valuable insights into metabolic disorders and potential treatment strategies.With its advanced technology and user-friendly protocols, the Rat GLP-1 CLIA Kit from Assay Genie is an essential tool for scientists working in the fields of diabetes, metabolism, and endocrinology. Trust in this kit to deliver precise and dependable results for your research needs.
Product Name:
Rat GLP-1 (Glucagon Like Peptide 1) CLIA Kit
SKU:
RTES00037
Target:
Rat GLP-1 (Glucagon Like Peptide 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GLP-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GLP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GLP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GLP-1. You can calculate the concentration of Rat GLP-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-103
87-104
87-101
Average (%)
97
95
94
1:4
Range (%)
89-99
87-100
86-99
Average (%)
94
92
92
1:8
Range (%)
96-113
88-100
103-119
Average (%)
103
94
110
1:16
Range (%)
88-102
102-117
90-106
Average (%)
96
110
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-102
95
EDTA plasma (n=5)
85-100
91
Cell culture media (n=5)
99-110
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.4
54.69
228.7
23.51
54.01
243.97
Standard deviation
1.88
5.86
26.16
1.92
4.2
24.13
C V (%)
8.03
10.71
11.44
8.17
7.78
9.89
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GLP-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GLP-1 in samples. No significant cross-reactivity or interference between Rat GLP-1 and analogues was observed.
