Rat Glial Cell Line Derived Neurotrophic Factor (GDNF) ELISA Kit

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SKU:
RTDL00427
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q07731
Sensitivity:
29pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
ATF1,ATF2,HFB1-GDNF,Astrocyte-Derived Trophic Factor,Glial Derived Neurotrophic Factor
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
Frequently bought together:

Description

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Rat Glial Cell Line Derived Neurotrophic Factor (GDNF) ELISA Kit

The Rat Glial Cell Line-Derived Neurotrophic Factor (GDNF) ELISA Kit is specifically designed for the precise quantification of GDNF levels in rat serum, plasma, and cell culture supernatants. This advanced kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.GDNF is a critical neurotrophic factor that plays a key role in promoting the survival and function of dopaminergic neurons, making it a valuable target for studying neurodegenerative disorders such as Parkinson's disease.

By accurately measuring GDNF levels, researchers can gain valuable insights into the mechanisms of neuroprotection and develop potential therapeutic interventions for these debilitating conditions.

Product Name:Rat Glial Cell Line Derived Neurotrophic Factor (GDNF) ELISA Kit
Product Code:RTDL00427
Size:96 Assays
Target:GDNF
Synonyms:ATF1, ATF2, HFB1-GDNF, Astrocyte-Derived Trophic Factor, Glial Derived Neurotrophic Factor
Detection Method:Sandwich
Range:78-5,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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