Rat GDF11 (Growth Differentiation Factor 11) CLIA Kit
The Rat GDF11 (Growth Differentiation Factor 11) CLIA Kit is a reliable and accurate assay designed for the quantitative measurement of GDF11 levels in rat serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.GDF11 is a key regulator of cell differentiation and development, playing a critical role in various biological processes such as skeletal muscle regeneration, neuroprotection, and aging.
Dysregulation of GDF11 has been associated with conditions like sarcopenia, Alzheimer's disease, and heart failure, highlighting its importance as a potential therapeutic target and diagnostic marker.With its advanced technology and superior performance, the Rat GDF11 CLIA Kit is an invaluable tool for scientists and researchers studying cellular differentiation, tissue regeneration, and age-related diseases in rat models.
Product Name:
Rat GDF11 (Growth Differentiation Factor 11) CLIA Kit
SKU:
RTES00268
Target:
Rat GDF11 (Growth Differentiation Factor 11)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GDF11. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GDF11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GDF11, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GDF11. You can calculate the concentration of Rat GDF11 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-109
102-116
97-108
Average (%)
99
109
102
1:4
Range (%)
102-119
101-118
88-103
Average (%)
110
109
95
1:8
Range (%)
89-100
93-111
98-115
Average (%)
95
101
105
1:16
Range (%)
99-114
84-99
95-105
Average (%)
105
91
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-112
102
EDTA plasma (n=5)
86-101
93
Cell culture media (n=5)
91-105
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.8
56.28
181.77
23.52
59.83
185.1
Standard deviation
2.45
5.09
17.78
2.85
7.13
14.22
C V (%)
9.88
9.04
9.78
12.12
11.92
7.68
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GDF11 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GDF11 in samples. No significant cross-reactivity or interference between Rat GDF11 and analogues was observed.
