The Rat Galectin-8 CLIA Kit (RTES00227) is a highly sensitive and specific assay designed to accurately measure Galectin-8 levels in rat serum, plasma, and cell culture supernatants. Galectin-8 is a key protein involved in various biological processes, including cell adhesion, inflammation, and immune response.This kit provides researchers with reliable and reproducible results, making it ideal for a wide range of research applications. Galectin-8 has been implicated in various diseases such as cancer, autoimmune disorders, and inflammatory conditions, making it a valuable biomarker for studying disease progression and potential therapeutic targets.
Overall, the Rat Galectin-8 CLIA Kit (RTES00227) is a powerful tool for investigating the role of Galectin-8 in physiological and pathological processes, providing valuable insights into disease mechanisms and therapeutic strategies.
Product Name:
Rat GAL8 (Galectin 8) CLIA Kit
SKU:
RTES00227
Target:
Rat GAL8 (Galectin 8)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GAL8. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GAL8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GAL8, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GAL8. You can calculate the concentration of Rat GAL8 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
100-118
91-102
Average (%)
104
107
96
1:4
Range (%)
92-106
98-114
91-104
Average (%)
98
107
98
1:8
Range (%)
98-113
98-114
85-96
Average (%)
105
106
91
1:16
Range (%)
94-108
99-113
98-110
Average (%)
100
107
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
101
EDTA plasma (n=5)
89-101
96
Cell culture media (n=5)
98-113
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
91.03
284.29
911.15
91.54
308.18
975.58
Standard deviation
8.59
23.28
72.07
9.47
34.64
86.83
C V (%)
9.44
8.19
7.91
10.35
11.24
8.9
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GAL8 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GAL8 in samples. No significant cross-reactivity or interference between Rat GAL8 and analogues was observed.
