The Rat FV (Coagulation Factor V) CLIA Kit is specifically designed for the precise measurement of Coagulation Factor V levels in rat plasma samples. This kit offers reliable and accurate results with high sensitivity and specificity, making it an ideal tool for researchers studying blood coagulation and related disorders.Coagulation Factor V is a key player in the blood clotting process, essential for maintaining hemostasis and preventing excessive bleeding. Abnormal levels of Factor V have been associated with various bleeding disorders and thrombotic diseases, highlighting its importance as a biomarker for diagnosing and monitoring these conditions.
With this Rat FV CLIA Kit, researchers can confidently assess Factor V levels in rat samples, facilitating a deeper understanding of coagulation mechanisms and potential therapeutic targets. Its user-friendly format and quick assay time make it a valuable asset for laboratories conducting coagulation research.
Product Name:
Rat FV (Coagulation Factor V) CLIA Kit
SKU:
RTES00134
Target:
Rat FV (Coagulation Factor V)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat F5. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat F5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat F5, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat F5. You can calculate the concentration of Rat F5 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-104
95-109
93-108
Average (%)
99
102
101
1:4
Range (%)
84-97
87-101
90-102
Average (%)
90
92
96
1:8
Range (%)
96-111
90-102
98-112
Average (%)
103
96
106
1:16
Range (%)
91-106
84-97
103-115
Average (%)
96
91
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-107
100
EDTA plasma (n=5)
92-102
97
Cell culture media (n=5)
86-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.04
5.69
15.91
2.13
5.54
16.63
Standard deviation
0.2
0.61
1.15
0.17
0.64
1.89
C V (%)
9.8
10.72
7.23
7.98
11.55
11.37
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat F5 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat F5 in samples. No significant cross-reactivity or interference between Rat F5 and analogues was observed.
