Rat Fractalkine Recombinant Protein (RPPB1138)

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SKU:
RPPB1138
Product type:
Recombinant Protein
Size:
20ug
Species:
Rat
Target:
Fractalkine
Synonyms:
Fractalkine
Synonyms:
CX3CL1
Synonyms:
Neurotactin
Synonyms:
CX3C membrane-anchored chemokine
Source:
Escherichia Coli
Uniprot:
O55145
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Description

Product Name:Rat Fractalkine Recombinant Protein
Product Code:RPPB1138
Size:20µg
Species:Rat
Target:Fractalkine
Synonyms:Fractalkine, CX3CL1, Neurotactin, CX3C membrane-anchored chemokine, Small inducible cytokine D1, NTN, NTT, CXC3, CXC3C, SCYD1, ABCD-3, C3Xkine.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.
Solubility:It is recommended to reconstitute the lyophilized Fractalkine in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized FractalkineRat although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Fractalkine should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.
Purity:Greater than 97.0% as determined by SDS-PAGE.
Amino Acid Sequence:QHLGMTKCNI TCHKMTSPIP VTLLIHYQLN QESCGKRAII LETRQHRHFC ADPKEKWVQD AMKHLDHQTA ALTRNG
Biological Activity:Determined by its ability to chemoattract human monocytes using a concentration range of 5.0-10.0 ng/ml, corresponding to a specific activity of 100,000-200,000units/mg.

Fractalkine soluble form is chemotactic for t-cells and monocytes, but not for neutrophils. Fractalkine membrane-bound form promotes adhesion of those leukocytes to endothelial cells. Fractalkine regulates leukocyte adhesion and migration processes at the endothelium and binds to CX3CR1. Natural Human Fractalkine is produced as a long protein (373-amino acid) with an extended mucin-like stalk and a chemokine domain on top. The mucin-like stalk permits it to bind to the cell surface. Fractalkine gene is located on human chromosome 16 along with some CC chemokines known as CCL17 and CCL22.

Fractalkine Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 76 amino acids and having a molecular mass of 8.7kDa.The Fractalkine is purified by proprietary chromatographic techniques.

UniProt Protein Function:Acts as a ligand for both CX3CR1 and integrins. Binds to CX3CR1 and to integrins ITGAV:ITGB3 and ITGA4:ITGB1. Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner. In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins. In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1. The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium.
NCBI Summary:chemokine that binds its receptor CX3CR1; induces chemotaxis and increased intracellular calcium levels in microglia [RGD, Feb 2006]
UniProt Code:O55145
NCBI GenInfo Identifier:18266784
NCBI Gene ID:89808
NCBI Accession:O55145.3
UniProt Related Accession:O55145
Molecular Weight:41,977 Da
NCBI Full Name:Fractalkine
NCBI Synonym Full Names:chemokine (C-X3-C motif) ligand 1
NCBI Official Symbol:Cx3cl1  
NCBI Official Synonym Symbols:Cx3c; Scyd1  
NCBI Protein Information:fractalkine; neurotactin; C-X3-C motif chemokine 1; small-inducible cytokine D1; CX3C membrane-anchored chemokine; small inducible cytokine subfamily D, 1
UniProt Protein Name:Fractalkine
UniProt Synonym Protein Names:C-X3-C motif chemokine 1; CX3C membrane-anchored chemokine; Neurotactin; Small-inducible cytokine D1Cleaved into the following chain:Processed fractalkine
Protein Family:Fractalkine
UniProt Gene Name:Cx3cl1  
UniProt Entry Name:X3CL1_RAT
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