Rat FNDC5 (Fibronectin type III domain-containing protein 5) ELISA kit
The Rat Fndc5 (Fibronectin Type III Domain-Containing Protein 5) ELISA Kit is specifically designed for the detection of Fndc5 levels in rat serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it an excellent tool for various research applications.Fndc5 is a protein that plays a key role in various physiological processes, including regulating metabolism, promoting muscle formation, and potentially impacting neurological functions.
Studies have shown that Fndc5 levels may be altered in conditions such as obesity, diabetes, and neurodegenerative diseases, making it a valuable biomarker for investigating these disorders and exploring potential therapeutic interventions.Overall, the Rat Fndc5 ELISA Kit offers researchers a reliable method for quantifying Fndc5 levels in rat samples, providing valuable insights into the role of this protein in health and disease.
Product Name:
Rat FNDC5 (Fibronectin type III domain-containing protein 5) ELISA kit
SKU:
RTES00910
Target:
Rat FNDC5 (Fibronectin type III domain-containing protein 5) ELISA kit
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat FNDC5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat FNDC5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat FNDC5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat FNDC5. You can calculate the concentration of Rat FNDC5 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
85-95
89-102
Average (%)
99
90
94
1:4
Range (%)
86-101
80-91
86-101
Average (%)
93
86
93
1:8
Range (%)
87-98
83-95
83-97
Average (%)
93
88
89
1:16
Range (%)
93-108
85-98
88-102
Average (%)
99
91
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-97
92
EDTA plasma (n=5)
88-101
94
Cell culture media (n=5)
88-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
202.57
612.64
1712.14
186.18
576.51
1563.28
Standard deviation
10.66
25.91
89.89
9.55
27.9
73.16
C V (%)
5.26
4.23
5.25
5.13
4.84
4.68
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat FNDC5 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat FNDC5 in samples. No significant cross-reactivity or interference between Rat FNDC5 and analogues was observed.
