Rat FLT1/VEGFR1 (Vascular Endothelial Growth Factor Receptor 1) ELISA Kit
The Rat FLT1 (VEGFR1) ELISA Kit is specifically designed for the quantitative measurement of Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) levels in rat serum, plasma, and tissue homogenates. This kit offers high sensitivity and accuracy, providing researchers with reliable and reproducible results for their studies.VEGFR1 is a key receptor involved in regulating angiogenesis, the process of forming new blood vessels. Dysregulation of VEGFR1 has been implicated in various diseases including cancer, diabetic retinopathy, and inflammatory disorders.
By accurately measuring VEGFR1 levels, researchers can gain insights into the role of this receptor in disease progression and potentially identify new therapeutic targets.Overall, the Rat FLT1 (VEGFR1) ELISA Kit is a valuable tool for researchers studying angiogenesis and vascular biology in rat models, providing a robust platform for exploring the role of VEGFR1 in various physiological and pathological conditions.
Product Name:
Rat FLT1/VEGFR1 (Vascular Endothelial Growth Factor Receptor 1) ELISA Kit
SKU:
RTES00756
Target:
Rat FLT1/VEGFR1 (Vascular Endothelial Growth Factor Receptor 1) ELISA Kit
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat VEGFR1/FLT1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat VEGFR1/FLT1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat VEGFR1/FLT1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat VEGFR1/FLT1. You can calculate the concentration of Rat VEGFR1/FLT1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-109
85-98
90-103
Average (%)
102
92
95
1:4
Range (%)
88-101
85-98
83-94
Average (%)
95
92
89
1:8
Range (%)
92-108
84-96
83-94
Average (%)
99
89
88
1:16
Range (%)
88-102
83-95
86-100
Average (%)
95
90
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-109
99
EDTA plasma (n=5)
85-97
91
Cell culture media (n=5)
89-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.55
1.28
3.82
0.54
1.24
4.03
Standard deviation
0.04
0.06
0.15
0.04
0.07
0.2
C V (%)
7.27
4.69
3.93
7.41
5.65
4.96
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat VEGFR1/FLT1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat VEGFR1/FLT1 in samples. No significant cross-reactivity or interference between Rat VEGFR1/FLT1 and analogues was observed.
