The Rat FIX (Coagulation Factor IX) CLIA Kit is a reliable and accurate assay designed for the quantitative detection of rat FIX levels in serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research applications.Coagulation Factor IX is a key protein involved in the blood clotting cascade, essential for maintaining hemostasis. Abnormal levels of FIX can lead to bleeding disorders such as hemophilia B.
This kit is ideal for studying coagulation disorders, evaluating treatment efficacy, and exploring potential therapeutic strategies for these conditions.Overall, the Rat FIX CLIA Kit offers a comprehensive solution for researchers looking to study coagulation factor IX in rat samples, providing valuable insights into hemostasis and potential therapeutic interventions.
Product Name:
Rat FIX (Coagulation Factor IX) CLIA Kit
SKU:
RTES00133
Target:
Rat FIX (Coagulation Factor IX)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat F9. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat F9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat F9, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat F9. You can calculate the concentration of Rat F9 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-103
99-112
84-96
Average (%)
97
106
90
1:4
Range (%)
90-104
96-110
98-113
Average (%)
97
103
106
1:8
Range (%)
100-116
98-114
100-118
Average (%)
107
104
107
1:16
Range (%)
86-98
97-110
98-113
Average (%)
91
102
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-105
99
EDTA plasma (n=5)
92-105
98
Cell culture media (n=5)
99-113
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
103.84
268.99
676.79
110.53
262.54
697.9
Standard deviation
12.69
28.49
56.85
11.04
30.56
47.81
C V (%)
12.22
10.59
8.4
9.99
11.64
6.85
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat F9 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat F9 in samples. No significant cross-reactivity or interference between Rat F9 and analogues was observed.
