Fibroblast growth factor receptor 4 (FGFR4) also known as CD334 antigen or tyrosine kinase related to fibroblast growth factor receptor, is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of FGFR4/CD334 interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. FGFR4/CD334 preferentially binds acidic fibroblast growth factor and, although its specific function is unknown, it is overexpressed in gynecological tumor samples, suggesting a role in breast and ovarian tumorigenesis. FGFR4/CD334 signaling is down-regulated by receptor internalization and degradation; MMP14 promotes internalization and degradation of FGFR4/CD334. Mutations in FGFR4/CD334 lead to constitutive kinase activation or impair normal FGFR4 inactivation lead to aberrant signaling.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
Product Name:
Rat FGFR4/CD334 Recombinant Protein (RPES1001)
Product Code:
RPES1001
Size:
50µg
Species:
Rat
Expressed Host:
HEK293 Cells
Synonyms:
FGFR-4
Accession:
Q498D6
Sequence:
Met 1-Asp 367
Fusion tag:
C-Fc
Activity:
1. Measured by its binding ability in a functional ELISA.2. Immobilized human FGF18 at 10 µg/mL (100 ?l/well) can bind Rat FGFR4, The EC50 of Rat FGFR4 is 1.17 µg/mL.3. Immobilized mouse FGF18 at 10 µg/mL (100 ?l/well) can bind Rat FGFR4, The EC50 of Rat FGFR4 is 0.44 µg/mL.4. Immobilized human bFGF at 10 µg/mL (100 ?l/well) can bind Rat FGFR4, The EC50 of Rat FGFR4 is 0.163 µg/mL.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method
Protein Construction:
A DNA sequence encoding the rat FGFR4 (Q498D6) extracellular domain (Met 1-Asp 367) was fused with the Fc region of human IgG1 at the C-terminus.
Purity:
> 90 % as determined by SDS-PAGE
Mol Mass:
66 kDa
AP Mol Mass:
110 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
