Based on the information from the provided URL (www.assaygenie.com/rat-fgf21-fibroblast-growth-factor-21-elisa-kit-rtes01036/), the Rat FGF21 (Fibroblast Growth Factor 21) ELISA Kit is specifically designed for the accurate measurement of FGF21 levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.FGF21 is a critical growth factor that plays a key role in regulating metabolic processes, including glucose and lipid metabolism.
It has been implicated in various metabolic disorders, such as obesity, diabetes, and liver diseases, making it a valuable biomarker for understanding these conditions and exploring potential therapeutic interventions.Overall, the Rat FGF21 ELISA Kit from Assay Genie is a reliable tool for researchers studying FGF21 biology and its implications in metabolic disorders, offering accurate and consistent results for their experiments.
Product Name:
Rat FGF21 (Fibroblast Growth Factor 21) ELISA Kit
SKU:
RTES01036
Target:
Rat FGF21 (Fibroblast Growth Factor 21)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat FGF21. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat FGF21 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat FGF21, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat FGF21. You can calculate the concentration of Rat FGF21 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-98
99-116
94-106
Average (%)
91
106
99
1:4
Range (%)
93-107
85-100
87-99
Average (%)
99
92
94
1:8
Range (%)
92-105
84-98
87-98
Average (%)
99
89
92
1:16
Range (%)
89-103
82-95
86-100
Average (%)
94
88
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-108
99
EDTA plasma (n=5)
85-99
91
Cell culture media (n=5)
89-99
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
105.59
243.96
934.7
99.66
224.36
983.34
Standard deviation
5.51
12.2
48.32
6.05
11.2
42.48
C V (%)
5.22
5.0
5.17
6.07
4.99
4.32
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat FGF21 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat FGF21 in samples. No significant cross-reactivity or interference between Rat FGF21 and analogues was observed.
