The Rat Ferritin Light Polypeptide (FTL) ELISA Kit is specifically designed for the precise quantification of FTL levels in rat serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of experimental needs.FTL is an important component of ferritin, a major iron storage protein responsible for regulating iron homeostasis and protecting cells from iron-induced oxidative damage.
Changes in FTL levels have been implicated in various disease states, including iron overload disorders, neurodegenerative diseases, and certain types of cancer. Therefore, the Rat FTL ELISA Kit is an invaluable tool for researchers studying these conditions and exploring novel therapeutic interventions.Order your Rat Ferritin Light Polypeptide (FTL) ELISA Kit today from Assay Genie and unlock new possibilities for your research endeavors.
Product Name:
Rat Ferritin, Light Polypeptide (FTL) ELISA Kit
Product Code:
RTDL00398
Size:
96 Assays
Target:
FTL
Synonyms:
Ferritin Light Chain, Ferritin Light Polypeptide-Like 3, L Apoferritin, Ferritin L Subunit
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
