Rat Fatty Acid Binding Protein 3, Muscle And Heart (FABP3) ELISA Kit

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SKU:
RTDL00353
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P07483
Sensitivity:
0.115ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
MDGI,FABP11,H-FABP,O-FABP,Hfabp,Mammary Derived Growth Inhibitor,Fatty Acid Binding Protein 11,Muscle fatty acid-binding protein,Heart-type fatty acid-binding protein
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
Frequently bought together:

Description

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Rat Fatty Acid Binding Protein 3, Muscle And Heart (FABP3) ELISA Kit

The Rat Fatty Acid Binding Protein 3 (FABP3) ELISA Kit is a reliable and accurate tool for measuring the levels of FABP3 in rat muscle and heart tissue samples. This kit has been specifically designed for use in research applications, offering high sensitivity and specificity to ensure precise and reproducible results.FABP3 is a key protein involved in the transport and metabolism of fatty acids, playing a crucial role in energy production and utilization in muscle and heart tissues.

Abnormal levels of FABP3 have been associated with various metabolic disorders and cardiovascular diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.With the Rat FABP3 ELISA Kit, researchers can confidently analyze FABP3 levels in rat samples with ease and accuracy, allowing for valuable insights into the role of this protein in physiological and pathological processes.

Product Name:Rat Fatty Acid Binding Protein 3, Muscle And Heart (FABP3) ELISA Kit
Product Code:RTDL00353
Size:96 Assays
Target:FABP3
Synonyms:MDGI, FABP11, H-FABP, O-FABP, Hfabp, Mammary Derived Growth Inhibitor, Fatty Acid Binding Protein 11, Muscle fatty acid-binding protein, Heart-type fatty acid-binding protein
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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